Audio level adjust

[DaveRook]

Periodically, I am informed that my microphone is a little quiet. 

I have unchecked "Automatically adjust microphone volume." (And this has been unchecked for the last year, and yet this issue has occurred multiple times during this period) 

This is where I want my microphone level to be

This is where it is being to 

As you can see, it's really a minor change but it does happen

I do have the Audio profile set to Medium

Is there anything outside of Zoom that might impact the level?